Method for recombinant production of heterologous polypeptide by making use of peptide exhibiting auto-proteolytic activity

摘要

The present invention relates to a process for the recombinant production of a desired heterologous polypeptide with a clearly defined homogeneous N-terminus in a bacterial host cell, wherein a fusion protein which comprises a peptide with the autoproteolytic activity of an autoprotease Nepro from a pestivirus and the heterologous polypeptide is formed in the form of cytoplasmic inclusion bodies in the host cell, and then the inclusion bodies are isolated and treated so that the desired heterologous polypeptide is cleaved autoproteolytically by the action of Nepro activity from the fusion protein.