Detection of Von-Willebrand factor (vwf) activity

摘要

The invention relates to a method for detecting von-Willebrand disease (vWD) comprising the steps of detecting von-Willebrand Factor (vWF) activity in a sample comprising a soluble form or portion of glycoprotein Ib(±) (GPIb(±)) and ristocetin botrocetin or a fragment thereof capable of inducing binding of vWF to GpIb(±), determining the amount of vWF-antigen in said sample, determining the ratio between vWF-activity and vWF-antigen for said sample, and comparing the above obtained ratio to the range of ratios established as normal range.
The invention further relates to the use of a kit comprising a soluble form or portion of glycoprotein Ib (±) (GPIb(±)) and ristocetin, botrocetin or a fragment thereof capable of inducing binding of vWF to GpIb(±), for detecting von - Willebrand disease (vWD).