METHOD FOR GREEN FLUORESCENT PROTEIN (GFP) SECRETION FROM PLANT CELLS

摘要

FIELD: medicine. ^ SUBSTANCE: constructed are recombinant plasmid DNA pAUTL-GFP bearing a hybrid gene coding green fluorescent protein (GFP) and a signal sequence of autolysin from Chlamydomonas reinhardtii. The produced plasmid is used to transform an Agrobacterium strain. A suspension of the transformed agrobacterial cells in a logarithmic growth stage is incubated with cells of Chlorella microalgae for 14-16 hours A protein secretion level is evaluated by measuring fluorescence of a culture environment. ^ EFFECT: new compounds show effective biological properties. ^ 2 cl, 1 dwg, 1 tbl, 2 ex