SYNTHETIC OLIGONUCLEOTIDE KIT FOR IDENTIFYING HUMAN MONOCYTIC EHRLICHIOSIS Ehrlichia spp AGENT DNA BY REAL-TIME POLYMERASE CHAIN REACTION

摘要

FIELD: medicine. ^ SUBSTANCE: invention refers to a synthetic oligonucleotide kit for identifying DNA of human monocytic ehrlichiosis (HME) agent - pathogenic representatives of Ehrlichia genus by a polymerase chain reaction. The offered invention can be used for the diagnostic purposes for detecting monocytic ehrlichiosis Ehrlichia spp. by the real-time polymerase chain reaction. Said kit includes primers as follows: 5'- GGG GAA AGA TTT ATC GCT ATT AG -3', 5'- CGG CAT AGC TGG ATC AGG CT -3' and a sample: (BHQl)-5'- CCC ACT GCT GCC (FdT)CC CGT AGG AGT CTG G - 3'P, where BHQ1 means a dark fluorescence killer attached to 5'-terminal nucleotide, while FdT is a fluorescent dye FAM attached to nucleotide T. ^ EFFECT: invention allows reliable identification of Ehrlichia representatives in the biological material. ^ 1 ex