| 摘要 |
FIELD: medicine. ^ SUBSTANCE: offered is a microorganism producing homosuccinic acid, of Mannheimia, Actinobacillus or Anaerobiospirillum genus. Said microorganism has been produced by destructing a lactate dehydrogenase (ldhA) coding gene, a phosphotransacetylase (pta) coding gene and an acetate kinase (ackA) coding gene, without destructing a pyruvate format lyase (pfl) coding gene. Besides, offered is a method for producing said microorganism and a method for producing succinic acid with applying thereof. The offered mutant microorganism exhibits a property of high growth rate and efficacy of succinic acid in the absence or low production of other organic acids, as compared to the previous strains producing succinic acid. ^ EFFECT: said microorganism is available for producing succinic acid for industrial application. ^ 13 cl, 7 dwg, 2 tbl, 5 ex |
