VIRUS CAPABLE OF EXPRESSING a-GALACTOSE EPITOPE, AND METHOD FOR PRODUCTION OF VACCINE

摘要

Disclosed are: a method for producing an a-Gal-expressing virus having enhanced immune response to viruses, without requiring the use of any enzyme; an influenza virus vaccine having a high effect (antigenicity), which is produced using an a-Gal-expressing virus produced by the method; and others. Specifically disclosed are: a method for producing an a-Gal-expressing virus, which comprises the steps of: (1) introducing an a1,3-galactose transferase gene into a cell system incapable of expressing an a-galactose epitope (Gala1-3Galß1-4GlcNAc-R: referred to as "a-Gal", hereinafter) in such a manner that the gene can be expressed in the cell system, thereby producing a cell system capable of expressing a-Gal; (2) inoculating a virus to the cell system capable of expressing a-Gal, thereby producing a virus-infected cell system; and (3) culturing the virus-infected cell system and obtaining a virus capable of expressing a-Gal from a culture solution; and a method for producing a vaccine, which comprises producing an a-Gal-expressing virus by the aforementioned method and preparing the vaccine from the resulting virus.