| 摘要 |
Materials and methods for preserving biological materials (e.g., organs, tissues, and cells) under cold or cryo conditions while reducing or minimizing damage to the materials. Methods can include placing an organ in a solution containing one or more of the following: 5 to 40 mM sorbitol, 5 to 120 mM potassium D-gluconate, 10 to 40 mM D-saccharic acid (potassium salt), 15 to 30 mM potassium phosphate monobasic, 3 to 12 mM potassium citrate monobasic, 8 to 12 mM magnesium chloride, 2 to 7 mM D-(+)-fructose, 1 to 6 mM glutathione(reduced), 3 to 7 mM adenosine, 20 to 40 mM sodium hydroxide, and 10 to 40mM potassium hydroxide, and having a pH of 7.3 to 7.5 and an osmolality of 290 to 360 mOsm/Kg. |
