发明名称 |
Methods of amplifying and sequencing nucleic acids |
摘要 |
This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; lighting the fragments to universal adapters; performing strand displacement and extension of the nicked fragments; purifying the double-stranded libation products; capturing the double-stranded libation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support. |
申请公布号 |
EP2261372(A3) |
申请公布日期 |
2011.04.06 |
申请号 |
EP20100179652 |
申请日期 |
2004.01.28 |
申请人 |
454 LIFE SCIENCES CORPORATION |
发明人 |
LEAMON, JOHN H.;LOHMAN, KENTON;ROTHBERG, JONATHAN M.;WEINER, MICHAEL P. |
分类号 |
C12Q1/68;B01L3/00;C07H21/00;C12N15/10;C12P19/34;G01N21/25;G01N21/64 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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