FLUORESCENT CYCLIC PEPTIDES, METHODS FOR THE PREPARATION THEREOF, AND USE OF SAID PEPTIDES FOR MEASURING THE ENZYMATIC ACTIVITY OF A PROTEASE ENZYME

摘要

The invention pertains to a cyclic peptide comprising the following sequence: -S1-X1-S2-X2- wherein S1 is a first target peptide sequence of a protease enzyme E1, S2 is a second target peptide sequence of a protease enzyme E2, S1 and S2 possibly being the same or different, said first peptide sequence S1 and said second peptide sequence S2 comprising 4 to 14 amino acids, E1 and E2 possibly corresponding to the same protease enzyme or to two different protease enzymes, X1 is a probe carrying a fluorescent donor group and X2 is a probe carrying a fluorescent or non-fluorescent acceptor group. Application of these peptides to determination of the activity of protease enzymes.