发明名称 |
CONSTRUCTING A DNA CHIMERA FOR VACCINE DEVELOPMENT AGAINST LEISHMANIASIS AND TUBERCULOSIS |
摘要 |
<p>A novel recombinant chimera of DNA construct having esat-6 region of Mycobacterium tuberculosis and kinesin region of Leishmania donovani cloned together on two sides of self cleaving peptide in a DNA vaccine vector pVAX-1 wherein the chimeric construct is operatively linked to a transcriptional promoter thus capable of self replication and expression within the mammalian cell, and the process of preparation thereof comprising: analysis of the predicted protein sequence of kinesin motor domain and esat-6 domain using Promiscuous MHC Class-1 Binding Peptide Prediction Servers; amplification of gene coding for kinesin motor domain and esat-6 domain; cloning of kinesin esat-6 gene region in pGEM-T™vector for sequence analysis; generation of chimeric construct by directional cloning in pVAX-1 vector. In-vitro expression analysis of kinesin motor domain and esat-6 domain from the clones using cell free translation system and immunogenicity studies; and splenocyte proliferation and cytokines studies using the above mentioned constructs.</p> |
申请公布号 |
EP2303316(A1) |
申请公布日期 |
2011.04.06 |
申请号 |
EP20090800156 |
申请日期 |
2009.02.10 |
申请人 |
DEPARTMENT OF BIOTECHNOLOGY;ALL INDIA INSTITUTE OF MEDICAL SCIENCES |
发明人 |
SINGH, SARMAN;DEY, AYAN |
分类号 |
A61K39/04;A61K39/008;C07K14/35;C07K14/44;C12N15/30;C12N15/31 |
主分类号 |
A61K39/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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