发明名称 |
Identification and use of genes encoding holins and holin-like proteins in plants for the control of microbes and pests |
摘要 |
This invention provides: 1) methods for the identification of broad-spectrum holins with a high level of nonenzymatic activity in membranes; 2) conditions required for maintaining and increasing the anti-microbial and anti-pest efficacy of holins in gene fusions; 3) a method for effective targeting of holins expressed in plants through use of a leader peptide to direct the holin protein to the plant apoplast and xylem; 4) methods for the control of bacterial and fungal diseases of plants and control of insect and nematode pests that attack plants by expression of gene fusions involving holins, C-terminal additions and leader peptides, and optionally, endolysins; 5) methods for increasing the shelf-life of cut flowers, and 6) transgenic plants useful for the production of novel antimicrobial proteins based upon holins.
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申请公布号 |
US7919601(B2) |
申请公布日期 |
2011.04.05 |
申请号 |
US20040556563 |
申请日期 |
2004.05.14 |
申请人 |
INTEGRATED PLANT GENETICS, INC.;UNIVERSITY OF FLORIDA RESEARCH FOUNDATION |
发明人 |
RAMADUGU CHANDRIKA;GABRIEL DEAN WILLIAM |
分类号 |
C07H21/04;C07K14/01;C12N;C12N7/00;C12N15/82 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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