摘要 |
<p>A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNTl-GaIT) comprising a CTS domain of N-acetylglucosaminyl transferase (GNTl) fused to a catalytic domain of beta-l,4galactosyltransferase (GaIT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.</p> |
申请人 |
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE;MEDICAGO INC.;UNIVERSITE DE ROUEN |
发明人 |
D'AOUST, MARC-ANDRE;MARQUET-BLOUIN, ESTELLE;BARDOR, MURIEL;BUREL, CAROLE;FAYE, LOIC;LEROUGE, PATRICE;VEZINA, LOUIS-PHILIPPE;GOMORD, VÉRONIQUE;AQUIN, STÉPHANIE;RIHOUEY, CHRISTOPHE;PACCALET, THOMAS;SOURROUILLE, CHRISTOPHE |