| 摘要 |
<P>PROBLEM TO BE SOLVED: To enable the rapid processing of clinical or biological samples, and the rapid and accurate detection of pathogens or antibiotic resistance genes in clinical samples. <P>SOLUTION: There are provided a rapid sample-processing method for preparing hybridization reaction mixtures substantially depleted of RNA, and a method of identifying the methicillin-resistance status and vancomycin-resistance status of an organism. Polynucleotide-based methods, compositions, kits, and devices are provided which can be used for detecting nucleic acids that encode resistance to methicillin and/or vancomycin. More specifically, the invention provides for detection of the mecA gene, which is associated with methicillin resistance of microorganisms, as well as for detection of the VanA and VanB genes, which are associated with vancomycin resistance of microorganisms. <P>COPYRIGHT: (C)2011,JPO&INPIT |
