METHOD FOR THE EXTRACTION AND DETECTION OF FAT-SOLUBLE COMPONENTS FROM BIOLOGICAL MATERIALS

摘要

The present invention relates to a method for the analysis of fat-soluble components, in particular dyes, from biological materials, in particular lipid rich foodstuffs, having an enrichment of the components and subsequent analysis. The method comprises a combination of extraction and separation steps and a subsequent analysis step. The invention further relates to an analytical kit and analytical equipment for carrying out the method. The method according to the invention is composed of a plurality of steps. The critical steps which are essential and characterize the invention are: 1.Pre-treatment of the sample to remove lipids. 2.Extracting the dyes into an extraction mixture by a specific solvent or solvent mixture. 3.Destruction of the oxidative sensible ingredients such as carotenoids prior to the final detection and quantification by eye or by optical enhancement.