| 摘要 |
Disclosed is ruggedized separation device (10) for analyzing a sample of bimolecular analyte using electrophoresis. The device includes: a test module (55); a holder (20) configured to support test module; an electrophoresis device (30) connected to the holder; a light source; a light detector; a plurality of optical devices; and a base plate onto which the light source, the light detector, and the plurality of optical devices are secured. The test module comprises one microfluidic channel disposed within a member formed of a transparent material, the microfluidic channel inelectrical communication with an anode (100) and a cathode (102). The microfluidic channel comprises: a separation portion; a waste arm in fluid communication with a waste arm opening, the waste arm opening is in fluid communication with a single separation portion; and a sample arm in fluid communication with a sample arm opening. The electrophoresis device comprises the anode and the cathode, and applies one or more biasing configurations to the test module. The light source emits a light beam that is transmitted to the test module and excites fluorescence in the sample of biomolecular analyte, the fluorescence being detected by the light detector. The optical devices transmit the light beam to the test module and the fluorescence from the test module to the light detector.
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