A process of purifying or enriching coagulation FVIII employing chromatography comprising the steps of providing a fraction containing FVIII in an aqueous solution having a high ionic strength; contacting the fraction containing FVIII with a multimodal resin; optionally washing the multimodal resin having FVIII adsorbed with an aqueous washing buffer; eluting FVIII containing fractions by an aqueous elution buffer comprising at least one amino acid which is positively charged at pH 6 to 8; and optionally collecting FVIII containing fractions in purified or enriched form.
申请公布号
EP2300497(A1)
申请公布日期
2011.03.30
申请号
EP20090769279
申请日期
2009.06.24
申请人
OCTAPHARMA AG
发明人
BORGVALL, CARIN;ERICSSON, ULRIKA;GILLJAM, GUSTAV;JERNBERG, MATS;WINGE, STEFAN