| 摘要 |
<p>A bacterial expression construct comprises a nucleic acid sequence encoding a secretion unit peptide comprising less than 300 amino acids of the C-terminus of a SPATE-class bacterial autotransporter polypeptide, the secretion unit peptide 5 comprising: (i) the alpha-helix; (ii) linker; and (iii) beta-barrel region of the beta-domain of the autotransporter polypeptide. Such an expression construct, and associated nucleic acids and peptides, find application in the expression of proteins of interest from a host bacterial cell to the cell culture medium.</p> |
