This invention relates to methods of generating single stranded DNA libraries for use in amplification and sequencing reactions. In various aspects, the disclosed methods include: fragmenting DNA; polishing the fragments' ends; lighting the fragments to universal adapters; performing strand displacement and extension of the nicked fragments; purifying the double-stranded libation products; capturing the double-stranded libation products onto a solid support; and isolating single stranded DNA library fragments, and binding these fragments to another solid support.
申请公布号
EP2145955(A3)
申请公布日期
2011.03.30
申请号
EP20090175588
申请日期
2004.01.28
申请人
454 CORPORATION
发明人
BERKA, JAN;CHEN, YI-JU;LEAMON, JOHN H.;LEFKOWITZ, STEVEN;LOHMAN, KENTON;MAKHIJANI, VINOD;SARKIS, GARY J.;ROTHBERG, JONATHAN;WEINER, MICHAEL;SRINIVASAN, MAITHREYAN