METHOD FOR THE DIFFERENTIATION OF ADIPOSE-DERIVED STEM CELLS INTO ADIPOCYTES USING HEPARIN-BINDING DOMAIN

摘要

PURPOSE: A method for differentiating adipose stem cells to adipocytes using heparin-binding domain(HBD) is provided to induce cell adhesion and to effectively obtain a large amount of adipocytes. CONSTITUTION: A culture container is fixed on a heparin-binding domain(HBD) with an adhesion activity of adipose stem cells. The HBD is fixed in a recombinant protein form with a polypeptide linker by physical absorption. The polypeptide linker is maltose-binding protein(MBP), hydrophobin, or hydrophobic cell penetrating peptides(CPPs). The recombinant protein has an amino acid sequence of sequence number 2.