| 发明名称 |
IMPROVED TRANSFECTION OF EUKARYOTIC CELL WITH LINEAR POLYNUCLEOTIDE BY ELECTROPORATION |
| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for more effective gene delivery into eukaryotic cells. <P>SOLUTION: The invention provides an improved method for gene delivery in eukaryotic cells by electroporation, preferably in human hematopoietic cells, in particular dendritic cells. The method is superior to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for gene delivery, including tumor antigen loading of dendritic cells. <P>COPYRIGHT: (C)2011,JPO&INPIT |
| 申请公布号 |
JP2011050395(A) |
申请公布日期 |
2011.03.17 |
| 申请号 |
JP20100265545 |
申请日期 |
2010.11.29 |
| 申请人 |
ANTWERPES INNOVATIECENTRUM:NV;SCHULER GEROLD |
发明人 |
SCHULER GEROLD;BERNEMAN ZWI N;VAN TENDELOO VIGGO F I;PONSAERTS PETER;STROBEL ISOLDE |
| 分类号 |
C12N15/09;A61K35/12;A61K38/00;A61K39/00;A61K48/00;A61L27/00;A61P35/00;A61P37/02;A61P43/00;C12N5/10;C12N15/87;C12P21/02;C12Q1/02;C12Q1/68 |
主分类号 |
C12N15/09 |
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