| 摘要 |
1,216,512. Insolubilized enzymes. BECKMAN INSTRUMENTS Inc. 10 Jan., 1968 [20 April, 1967], No. 1465/68. Heading C3H. A protein is coupled to a compound which is itself coupled to a substrate via a diazo linkage by (a) coupling a substrate to a diazonium compound which contains a group which does not react in the diazotization reaction and is capable of reacting with a protein, and (b) coupling a protein via said group to the product of step (a). The invention also comprises a method of insolubilizing an enzyme by (a) coupling a protein to a compound which has itself been coupled to a substrate via a diazo linkage, and (b) coupling the enzyme to the protein. The substrates may be porous, e.g. polyurethane foam. The diazo compound may be formed from p-aminohippuric acid, p-amino-phenylacetic acid, 4-pamino - phenylbutyric acid, p - amino - benzoic acid, p-amino-phenoxyacetic acid or p-aminophenylalanine. The protein may be human serum albumin, ovalbumin, bovine collagen or casein. Examples are given of insolubilized glucose oxidase. The polyurethane may be formed by the reaction with toluene di-isocyanate and water of a hydroxyl terminated polyester resin condensed from adipic acid, diethylene glycol and trimethylol propane. |
