Active-site engineering of nucleotidylyltransferases and general enzymatic methods for the synthesis of natural and“unnatural”UDP- and TDP-nucleotide sugars

摘要

The present invention provides mutant nucleotidylyl-transferases, such as Ep, having altered substrate specificity; methods for their production; and methods of producing nucleotide sugars, which utilize these nucleotidylyl-transferases. The present invention also provides methods of synthesizing desired nucleotide sugars using natural and/or modified Ep or other nucleotidyltransferases; and nucleotide sugars synthesized by the present methods. The present invention further provides new glycosyl phosphates, and methods for making them.