METHOD AND KIT FOR MEASURING ENZYMATIC ACTIVITIES OF VARIOUS CYTOCHROME P450 MOLECULE SPECIES COMPREHENSIVELY AND WITH HIGH EFFICIENCY

摘要

A laminated substrate comprising an oxygen sensor layer and a cytochrome P450-supported layer both laminated on a substrate, wherein cytochrome P450 is supported on a hydrophilic polymer carrier; at least one caged compound selected from the group consisting of caged NADP and caged G6P; a method for measuring the enzymatic activity of P450 comprehensively and with high efficiency and high precision, which is characterized in that the reaction between an enzyme utilizing NADPH as a coenzyme (i.e., a cytochrome P450 reductase) and a substrate for the enzyme is initiated upon the production of NADPH by irradiating the caged compound with light in the co-presence of the enzyme and the substrate to supply NADP and/or G6P from the caged compound; and a measurement kit.