RESTRICTION ENDONUCLEASE VARIANTS WITH ALTERED CLEAVAGE SPECIFICITY AND/OR REDUCTION OF STAR ACTIVITY

摘要

Mutations introduced into a restriction endonuclease may alter not only fidelity of cleavage but also specificity. Examples of novel variant endonucleases with altered specificities are described and also variants with high fidelity of cleavage. Examples describe mutations in Btsl that result in at least one of altered cleavage and nicking and high fidelity cleavage.