METHOD FOR DIFFERENTIATION OF STEM CELLS INTO VASCULAR CELLS AND THE INDUCTION OF ANGIOGENESIS USING THE SAME
摘要
The present invention relates to a method for differentiating stem cells into vascular cells by culturing them in the form of a three-dimensional cell cluster and the use of the three-dimensional cell cluster for angiogenesis. Specifically, the present invention teaches a method for differentiating stem cells into vascular cells comprising culturing stem cells by adhering them onto a culture plate with a surface having a hydrophobic property or a culture plate onto which a growth factor is immobilized, wherein the cultured stem cells are later detached from the culture plate as their density increases to form a three-dimensional cell cluster and grown in the form of a three-dimensional cell cluster while differentiating into vascular cells. Also disclosed is the use of a three-dimensional cell cluster composed of the vascular cells differentiated from stem cells by the above method as a cell therapy agent for angiogenesis.
申请公布号
WO2010110596(A3)
申请公布日期
2011.03.10
申请号
WO2010KR01807
申请日期
2010.03.24
申请人
KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY;KIM, SANG-HEON;KIM, SOO HYUN;PARK, IN SU;JUNG, YOUNG MEE
发明人
KIM, SANG-HEON;KIM, SOO HYUN;PARK, IN SU;JUNG, YOUNG MEE