发明名称 |
MISCHZELL-POPULATIONEN FÜR DIE GEWEBEREPARATUR UND TRENNTECHNIK FÜR DIE ZELLAUFBEREITUNG |
摘要 |
The present invention provides a method for processing cultured cells. The method includes the steps of providing a biochamber for culturing cells; providing a culture media for culturing cells within the biochamber; inoculating the biochamber with cells; culturing the cells; upon a predetermined time period of culture, displacing the culture media from the biochamber with a biocompatible first rinse solution; replacing the first rinse solution with a cell harvest enzyme solution; incubating the contents of the biochamber for a predetermined period of time, wherein during incubation, the enzyme at least dissociates the cells from each other and/or from the biochamber surface; and displacing the enzyme solution with a second rinse solution, wherein upon the enzyme being displaced, the chamber is substantially filled with the second rinse solution. |
申请公布号 |
DE602007012290(D1) |
申请公布日期 |
2011.03.10 |
申请号 |
DE20076012290T |
申请日期 |
2007.11.05 |
申请人 |
AASTROM BIOSCIENCES INC. |
发明人 |
HAMPSON, BRIAN;GOLTRY, KRISTIN;SMITH, DOUGLAS M.;ROWLEY, JONATHAN A.;VENTURI, NAIA |
分类号 |
A61K35/12;A61P9/10;C12N5/078 |
主分类号 |
A61K35/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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