| 摘要 |
A method of preparing DNA fragments, which entails at least the following steps of: a) preparing double-stranded DNA fragments from a sample of nucleic acids to be analyzed, b) ligating the ends of said DNA fragments to a double-stranded oligonucleotide adaptor (adaptor AA′) comprising the recognition site for a restriction enzyme, the cleavage site of which is located downstream of said recognition site, c) amplifying the fragments linked to said adaptor, using a pair of suitable primers, at least one being optionally labeled at its 5′ end, and d) cleaving said DNA fragments close to one of their ends, using said restriction enzyme, so as to generate short fragments.
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