发明名称 |
High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences |
摘要 |
A purified and isolated DNA sequence has protein production increasing activity. Said DNA sequence comprises at least one bent DNA element, and at least one binding site for a DNA binding protein. It is a MAR nucleotide with the sequence SEQ ID No 26, a sequence complementary thereof, or a sequence having at least 70% identity with said sequence. Advantageously the bent DNA element contains at least 10% of dinucleotide TA, and/or at least 12% of dinucleotide AT on a stretch of 100 contiguous base pairs. |
申请公布号 |
EP2292754(A1) |
申请公布日期 |
2011.03.09 |
申请号 |
EP20100178871 |
申请日期 |
2004.10.22 |
申请人 |
SELEXIS S.A. |
发明人 |
MERMOD, NICOLAS;GIROD, PIERRE ALAIN;BUCHER, PHILIPP;CALABRESE, DAVID;NGUYEN, DUC-QUANG;SAUGY, DAMIEN;PUTTINI, STEFANIA |
分类号 |
C12N15/10;C12N15/11;A01K67/027;C12N5/10;C12N15/63;C12N15/67;C12N15/79;C12N15/87;G06F19/00 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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