METHODS OF BEAD MANIPULATION AND FORMING BEAD ARRAYS
摘要
<p>According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.</p>
申请公布号
WO2011026102(A1)
申请公布日期
2011.03.03
申请号
WO2010US47308
申请日期
2010.08.31
申请人
LIFE TECHNOLOGIES CORPORATION;OLDHAM, MARK;FRY, GEORGE;INMAN, CHRISTINA;BRIDGHAM, JOHN;HUNKAPILLAR, TIMOTHY;VANN, CHARLES
发明人
OLDHAM, MARK;FRY, GEORGE;INMAN, CHRISTINA;BRIDGHAM, JOHN;HUNKAPILLAR, TIMOTHY;VANN, CHARLES