| 摘要 |
A method for detecting an objective substance in a sample using a chromatographic test device having a detection region on which a capture substance capable of binding to the objective substance is immobilized, comprising: mixing the sample and an enzyme-labeled substance capable of binding to the objective substance; trapping the objective substance binding to the enzyme-labeled substance by the capture substance in the detection region, by adding the liquid mixture to the chromatographic test device; adding an anionic surfactant-containing reagent to the detection region in which the objective substance is captured; reacting a luminescent substrate with the enzyme of the enzyme-labeled substance binding to the capture substance through the objective substance in the detection region; and detecting the objective substance in the sample by detecting luminescence generated from the detection region by the reaction of the luminescent substrate with the enzyme. A kit is also disclosed.
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