METHOD OF AND APPARATUS FOR IMAGING A CELLULAR SAMPLE

摘要

A method of imaging a cellular sample by multi-photon imaging, the method comprising: providing a multi-photonic excitation beam; splitting the multi-photonic excitation beam into a plurality of N sub-beams; modulating the amplitude of the plurality of N sub-beams to create N respective temporal modulation pattern; focusing the plurality of N modulated sub-beams to N respective spatially separated focal points P1... PN within the cellular sample; simultaneously detecting fluorescent signals emitted from the N focal points P1... PN; and demodulating the detected fluorescent signals so that fluorescence data relating to the respective focal points P1...PN may be obtained. An apparatus for imaging a cellular sample is also described.