METHOD OF CONCENTRATING HUMAN MESENCHYMAL STEM CELLS

摘要

The present invention is intended to provide methods for highly enriching human mesenchymal stem cells from a cell population containing the human mesenchymal stem cells. To highly enrich human mesenchymal stem cells, CD271+CD90+ cells are recovered by using flow cytometry etc. from a cell population containing the human mesenchymal stem cells. If the cell population contains blood cells (as in the case of a cell population prepared from a bone marrow, a peripheral blood etc.), CD45−CD235a−CD271+CD90+ cells are recovered. These cell fractions contain with high purity the mesenchymal stem cells having self-renewal capability, self-replicating capability and pluripotency. Therefore, human mesenchymal stem cells can be highly enriched by recovering CD271+CD90+ cells from the cell population containing the human mesenchymal stem cells.