| 摘要 |
PROBLEM TO BE SOLVED: To provide compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. SOLUTION: The compositions comprise one or more nitrogen-containing organic compounds selected from the group consisting of specific constitutional formulae (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprises one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-methylimidazole. The invention further relates to methods for high-fidelity synthesis of nucleic acid molecules, including via amplification, reverse transcription, and sequencing. The invention also relates to nucleic acid molecules synthesized by these methods or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing, or sequencing nucleic acid molecules comprising one or more of the compositions. COPYRIGHT: (C)2011,JPO&INPIT
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