METHOD OF GENERATING INFORMATIVE DNA TEMPLATES FOR HIGH-THROUGHPUT SEQUENCING APPLICATIONS

摘要

Methods and compositions are disclosed for generating informative DNA templates for sequencing. The methods generally include fragmenting an individual's genomic DNA to generate templates with predetermined range of sequence lengths, ligating adapters; selecting informative DNA templates, which contain a polymorphism; and recovering enriched informative DNA templates. The DNA templates may then be sequenced using a high throughput sequencing process, and the resulting sequences compared to a reference set of sequences obtained from the genomes of other individuals, to identify DNA polymorphisms and to generate genotypes.