Provided is a mutant Pichia pastoris alcohol oxidase 1 (AOXl) promoter of the wild type Pichia pastoris AOXl promoter with a specified sequence comprising at least one mutation within nucleotides 694 to 723 for high expression under derepression conditions. Further variants with one other additional mutation at specific advantageous locations are also disclosed. The promoter can be used for efficient expression of desired recombinant proteins. (62) Divided Out of 560542
