OPTICAL MICROSCOPY USING PHOTOTRANSFORMABLE OPTICAL LABEL

摘要

PROBLEM TO BE SOLVED: To provide an optical microscopy using phototransformable optical labels.

SOLUTION: First activation radiation is provided to a sample 601 that includes phototransformable optical labels ("PTOLs") to activate a first subset of the PTOLs in the sample. First excitation radiation is provided to the first subset of PTOLs in the sample to excite at least some of the activated PTOLs, and radiation emitted from activated and excited PTOLs within the first subset of PTOLs is detected by imaging optical elements 606. The first activation radiation is controlled such that the mean volume per activated PTOLs in the first subset is greater than or approximately equal to a diffraction-limited resolution volume ("DLRV") of the imaging optical elements 606.

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