VIRAL VECTOR CONTAINING RECOMBINATION SITE

摘要

PROBLEM TO BE SOLVED: To provide compositions and methods for the construction of nucleic acids comprising all or a portion of a viral genome.

SOLUTION: The recombinant viral genome may be constructed to contain multiple recombination and/or topoisomerase recognition sites. The compositions include vectors having multiple recombination sites with unique specificity that contain all or a portion of a viral genome. The methods permit the insertion of a sequence of interest into a viral genome using recombinational and/or topoisomerase-mediated cloning. There are also provided methods of constructing recombinant virus, methods of expressing polypeptides, and methods of expressing fusion polypeptides.

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