| 摘要 |
Provided are surprisingly beneficial osmotic stress shock methods for facilitating disruption and/or extraction of microorganisms, comprising a synergistic combination of at least one hypotonic shock and at least two hypertonic shocks, wherein the last shock is a hypertonic shock. Preferred aspects of the invention relate to facilitating disruption of a broad spectrum of microorganisms (e.g., algae, bacteria, yeast, fungus, etc.). In particular aspects the microbial cells are subjected to: a hypertonic/hypotonic/hypertonic tertiary shock; a hypotonic/hypertonic/hypertonic tertiary shock; or a hypotonic/hypertonic/hypotonic/hypertonic quaternary shock. Particular aspects further comprise disrupting and/or extracting of the shocked microbial cells, and isolating a cellular constituent or bioproduct (e.g., biofuel, biocrude, bioenergy, biogas, biodiesel, bioethanol, biogasoline, pharmaceuticals, nutraceuticals, food, vitamins, feedstock, dyes, colorants, sulfur, fertilizer, bioplastic) therefrom. In particular preferred aspects, facilitation of algae disruption and/or extraction is provided and in certain embodiments, isolation of at least one of lipid, oil, and triacylglycerol is enhanced.
|
