EURAKYOTIC SECRETION SIGNAL SEQUENCE FOR RECOMBINANT EXPRESSION IN PROKARYOTES
摘要
<p>An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.</p>
申请公布号
WO2009147382(A3)
申请公布日期
2011.02.24
申请号
WO2009GB01372
申请日期
2009.06.01
申请人
MSD BIOLOGICS (UK) LIMITED;KARA, BHUPENDRA, VALLABH;LENNON, CHRISTOPHER, DAVID, JOHN
发明人
KARA, BHUPENDRA, VALLABH;LENNON, CHRISTOPHER, DAVID, JOHN