Production of and uses for decellularized lung tissue

摘要

The present invention provides a process of producing a decellularized extracellular matrix DC lung from native lung tissue using rapid freeze/thaw cycling to induce cellular damage and the constant circulation of a detergent or peracetic acid and enzymatic digestion with DNAase/RNAase within a continuously rotating bioreactor. Also, provided are methods to produce a functional engineered lung tissue on the DC lung using endogenous lung progenitor cells. In addition, a composition comprising the DC lung and the endogenous lung progenitor cells seeded therein or thereon and an implantable composition comprising the functional engineered lung tissue which are useful in methods of treating a lung to restore at least some function thereto.