MULTI-SPOT METAL-CAPPED NANOSTRUCTURE ARRAY NUCLEIC ACID CHIP FOR DIAGNOSIS OF CORNEAL DYSTROPHY AND PREPARING METHOD THEREOF

摘要

PURPOSE: A multi-spot metal-capped nanostructure array nucleic acid chip for diagnosing corneal dystrophy is provided to apply as an LSPR optical property-based non-labeled optical biosensor and to diagnose various corneal dystrophy at once. CONSTITUTION: A method for manufacturing a multi-spot metal-capped nanostructure array nucleic acid chip comprises: a step of forming a metal thin film(second layer) on a substrate(first layer); a step of forming multi-spot on the metal thin film and arranging nanostructure on each spot surface in constant interval to form a nanostructure layer(third layer); a step of forming the metal thin film on the nanostructure layer; and a step of fixing a probe nucleic acid on the metal thin film(fourth layer).