| 摘要 |
<p>A method of generating and isolating recombinant high affinity anti-sialic acid antibody molecules comprises the steps of immunising an avian host with an immunogen comprising a conjugate of sialic acid and a carrier protein to generate an anti-sialic acid polyclonal serum, isolating a sample of RNA from the immunised avian host, using phage display in the generation and screening of a library of recombinant avian antibody molecules from the RNA sample, and isolating recombinant high affinity anti-sialic acid antibody molecules. The antibody molecule is selected from the group consisting of: whole antibodies; scFv fragments; and Fab fragments, and the avian host is Gallus domesticus. A recombinant avian antibody fragment having high binding affinity to sialic acid and obtainable by the method of the invention, and an anti-sialic acid polyclonal serum obtainable by immunising an avian host with a conjugate of sialic acid and carrier protein, are also described.</p> |
