发明名称 |
High affinity TCR proteins and methods |
摘要 |
T cell receptors (TCRs) that have higher affinity for ligand than wild type TCRs are provided. These high affinity TCRs are formed by mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants of the T cell receptor protein coding sequence; transforming the T cell receptor mutant coding sequence into yeast cells; inducing expression of the T cell receptor mutant coding sequence on the surface of yeast cells; and selecting those cells expressing T cell receptor mutants that have higher affinity for the peptide/MHC ligand than the wild type T cell receptor protein. The high affinity TCRs can be used in place of an antibody or single chain antibody. |
申请公布号 |
EP2287185(A2) |
申请公布日期 |
2011.02.23 |
申请号 |
EP20100184889 |
申请日期 |
2000.12.06 |
申请人 |
BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS |
发明人 |
KRANZ, DAVID M.;HOLLER, PHILLIP D.;WITTRUP, K. DANE |
分类号 |
G01N33/53;G01N33/536;A61K39/00;A61K39/38;C07K14/725;C07K14/74;C12N5/10;C12N15/09;C12Q1/02;G01N33/566;G01N33/58 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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