发明名称 |
RAPID AND QUANTITATIVE PROTEOME ANALYSIS AND RELATED METHODS |
摘要 |
The invention provides methods for identifying polypeptides by simultaneously determining the mass of a subset of parent polypeptides from a population of polypeptides and the mass of fragments of the subset of parent polypeptides; comparing the determined masses to an annotated polypeptide index; and identifying one or more polypeptides of the annotated polypeptide index having the determined masses. The invention also provides methods for identifying a polypeptide by determining two or more characteristics associated with the polypeptide, or a fragment thereof, one of the characteristics being mass of a fragment of the polypeptide, the fragment mass being determined by mass spectrometry; comparing the characteristics associated with the polypeptide to an annotated polypeptide index; and identifying one or more polypeptides in the annotated polypeptide index having the characteristics. The method can additionally include the step of quantitating the amount of the identified polypeptide in a sample containing the polypeptide.
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申请公布号 |
CA2433281(C) |
申请公布日期 |
2011.02.22 |
申请号 |
CA20012433281 |
申请日期 |
2001.12.21 |
申请人 |
THE INSTITUTE FOR SYSTEMS BIOLOGY;UNIVERSITY OF WASHINGTON |
发明人 |
GOODLETT, DAVID R.;AEBERSOLD, RUDOLF H. |
分类号 |
G01N27/62;G01N33/48;C12N15/09;G01N30/88;G01N33/483;G01N33/68 |
主分类号 |
G01N27/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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