The present invention relates to methods for ligation. The invention provides novel reagents and methods for ligating an acyl donor compound with an acyl acceptor compound. Provided acyl donor compounds comprise a transamidase recognition sequence that allows ligation with a nucleophilic acyl acceptor in the presence of transamidase. The invention further provides kits comprising acyl donor compounds and optionally comprising other reagents for ligation.
申请公布号
WO2010087994(A3)
申请公布日期
2011.02.17
申请号
WO2010US00274
申请日期
2010.02.01
申请人
WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH;PLOEGH, HIDDE, L.;ANTOS, JOHN, M.;POPP, MAXIMILIAN, WEI-LIN;GUIMARAES, CARLA