| 摘要 |
The present invention provides methods for selectively amplifying a target population of nucleic acid molecules in a population of RNA template molecules (e.g., all mRNA molecules expressed in a cell type except for the most highly expressed mRNA species). The present invention also provides a first population of oligonucleotides including the nucleic acid sequences set forth in SEQ ID NOS:1-749 and a second population of oligonucleotides including the nucleic acid sequences set forth in SEQ ID NOS:750-1498. The first population of oligonucleotides can be used, for example, to prime the synthesis of first strand cDNA molecules complementary to mRNA molecules isolated from mammalian cells without priming the synthesis of cDNA molecules complementary to ribosomal RNA molecules. The second population of oligonucleotides can be used, for example, to prime the second strand synthesis of primer extension products (first strand cDNA) complementary to mRNA molecules isolated from mammalian cells without priming the second strand synthesis of primer extension products synthesized from ribosomal RNA molecules.
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