The invention is directed to a system for performing multi-color real time PCR, comprising a flexible real time PCR instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.
申请公布号
EP2284278(A1)
申请公布日期
2011.02.16
申请号
EP20100170020
申请日期
2004.04.01
申请人
ROCHE DIAGNOSTICS GMBH;F. HOFFMANN-LA ROCHE AG
发明人
BECHLER, INGRID;BOLTE, JOCHEN;GUTEKUNST, MARTIN;HEINDL, DIETER;JOSEL, HANS-PETER;MUELLER, CHRISTOPH;SAGNER, GREGOR;SEIBL, RUDOLF