Fusion proteins useful for detecting analytes

摘要

Disclosed is a fusion protein comprising:
(a) two detection portions, wherein
(i) the first detection portion is an energy-emitting protein portion and the second detection portion is a fluorescent protein portion;
or
(ii) the two detection portions are portions of a split fluorescent protein; and
(b) a periplasmic binding protein (PBP) portion between said two detection portions, which undergoes a conformational change upon binding of a compound;
wherein said conformational change results in a change of the energy emitted by said two detection portions.