| 摘要 |
The present teaching generally discloses a method of measuring the concentration of an analyte in a solution. The method includes selecting a binding compound; selecting an analog to an analyte and conjugating the analog to a polynucleotide substrate to construct a reagent for measuring the amount of the analyte in an assay solution using a desired polynucleotide reaction that comprises a polymerization reaction, a cleavage reaction, or a recombination reaction. The binding of the reagent to the binding compound in the assay solution competes with the binding of the analyte to the binding compound in the assay solution. The method also includes selecting a desired enzyme for catalyzing the desired polynucleotide reaction. Reagents and kits are also provided.
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