发明名称 |
Methods and compositions for targeted cleavage and recombination |
摘要 |
Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins. |
申请公布号 |
US7888121(B2) |
申请公布日期 |
2011.02.15 |
申请号 |
US20040912932 |
申请日期 |
2004.08.06 |
申请人 |
SANGAMO BIOSCIENCES, INC. |
发明人 |
URNOV FYODOR;HOLMES MICHAEL C.;MILLER JEFFREY C.;PABO CARL O. |
分类号 |
C12N15/00;C07H21/04;C07K14/435;C07K14/47;C07K14/715;C12N9/14;C12N9/22;C12N15/62;C12N15/79;C12N15/82;C12N15/85;C12N15/86;C12N15/861;C12P19/34;C12Q1/68 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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